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LTA4H Polyclonal Antibody

CAC15053
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLTA4H
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Overview

  • Supplier
    Biomatik
  • Product Name
    LTA4H Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4048
  • Target name
    LTA4H
  • Target description
    leukotriene A4 hydrolase
  • Target synonyms
    leukotriene A-4 hydrolase; LTA-4 hydrolase; testicular secretory protein Li 27; tripeptide aminopeptidase LTA4H
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09960
  • Protein Name
    Leukotriene A-4 hydrolase
  • Scientific Description
    The LTA4H Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LTA4H using anti-LTA4H antibody (A02399-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat thymus tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTA4H antigen affinity purified polyclonal antibody (Catalog # A02399-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTA4H at approximately 69 kDa. The expected band size for LTA4H is at 69 kDa.
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