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ProductsBack/anti-Lysozyme antibody [SB1 (BGN/06/961)]

anti-Lysozyme antibody [SB1 (BGN/06/961)]

ARG23896
Arigo Biolaboratories
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLYZ
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-Lysozyme antibody [SB1 (BGN/06/961)]
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SB1 (BGN/06/961)
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4069
  • Target name
    LYZ
  • Target description
    lysozyme
  • Target synonyms
    1,4-beta-N-acetylmuramidase C; c-type lysozyme; lysozyme C; lysozyme F1; LYZF1; LZM
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (A01811-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lysozyme/LYZ antigen affinity purified polyclonal antibody (Catalog # A01811-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lysozyme/LYZ at approximately 15-17 kDa. The expected band size for Lysozyme/LYZ is at 17 kDa.
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