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Lysozyme antibody

10R-L110A

Product group Antibodies

Overview

Supplier
Biosynth
Product Name
Lysozyme antibody
Delivery Days Customer
11
Certification
Research Use Only
UNSPSC
41116161

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Figure 1. Western blot analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (A01811-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lysozyme/LYZ antigen affinity purified polyclonal antibody (Catalog # A01811-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lysozyme/LYZ at approximately 15-17 kDa. The expected band size for Lysozyme/LYZ is at 17 kDa.

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$Paraformaldehyde-fixed, paraffin embedded mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Lysozyme Polyclonal Antibody, Unconjugated (bs-0816R) at 1:200 overnight at 4°C, followed by a conjugated \nsecondary for 90 minutes and DAB staining.$

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