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Immunohistochemical staining of human lymph node shows moderate positivity in nuclear membrane in germinal center cells.
Immunohistochemical staining of human lymph node shows moderate positivity in nuclear membrane in germinal center cells.
Immunohistochemical staining of human lymph node shows moderate positivity in nuclear membrane in germinal center cells.

Anti-MAD1L1 Antibody

HPA003635
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAD1L1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAD1L1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8379
  • Target name
    MAD1L1
  • Target description
    mitotic arrest deficient 1 like 1
  • Target synonyms
    MAD1; MAD1 mitotic arrest deficient like 1; MAD1-like protein 1; mitotic arrest deficient 1-like protein 1; mitotic checkpoint MAD1 protein homolog; mitotic spindle assembly checkpoint protein MAD1; mitotic-arrest deficient 1, yeast, homolog-like 1; PIG9; tax-binding protein 181; TP53I9; tumor protein p53 inducible protein 9; TXBP181
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6D9
  • Protein Name
    Mitotic spindle assembly checkpoint protein MAD1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MAD1 using anti-MAD1 antibody (PB9262). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. Lane 5: human HL-60 whole cell lysates. Lane 6: human THP-1 whole cell lysates. Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD1 antigen affinity purified polyclonal antibody (Catalog # PB9262) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAD1 at approximately 83KD. The expected band size for MAD1 is at 83KD.
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