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Anti-Mad3 [N129A/6R]

Ab02313-10.0
Absolute Antibody
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
TargetBUB1B
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Mad3 [N129A/6R]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is recommended for detection and analysis of Mad3 by Immunocytochemistry and western blot. For instance, the mouse version of this antibody was used to detect Mad3 by western blot in extracts of COS cells transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3, respectively) and untagged Kv2.1 plasmids.
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    N129A/6R
  • Gene ID701
  • Target name
    BUB1B
  • Target description
    BUB1 mitotic checkpoint serine/threonine kinase B
  • Target synonyms
    Bub1A; BUB1B, mitotic checkpoint serine/threonine kinase; BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 homolog beta; hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; mitotic checkpoint kinase MAD3L; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; SSK1
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDO60566
  • Protein Name
    Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    human, mouse
  • Reactivity Supplier Note
    This antibody was raised against a fusion protein of amino acids 116-206 of human Mad3.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BubR1/BUB1B antigen affinity purified polyclonal antibody (Catalog # A01564-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BubR1/BUB1B at approximately 120-130KD. The expected band size for BubR1/BUB1B is at 120-130KD.
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