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Western Blot analysis of HeLa cells using BubR1 Polyclonal Antibody
Western Blot analysis of HeLa cells using BubR1 Polyclonal Antibody
Western Blot analysis of HeLa cells using BubR1 Polyclonal Antibody

BUB1B Antibody

CSB-PA001071
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetBUB1B
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Overview

  • Supplier
    Cusabio
  • Product Name
    BUB1B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID701
  • Target name
    BUB1B
  • Target description
    BUB1 mitotic checkpoint serine/threonine kinase B
  • Target synonyms
    Bub1A; BUB1B, mitotic checkpoint serine/threonine kinase; BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 homolog beta; hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; mitotic checkpoint kinase MAD3L; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; SSK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60566
  • Protein Name
    Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BubR1/BUB1B antigen affinity purified polyclonal antibody (Catalog # A01564-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BubR1/BUB1B at approximately 120-130KD. The expected band size for BubR1/BUB1B is at 120-130KD.
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