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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatogonia.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatogonia.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatogonia.

Anti-MAGEC3 Antibody

HPA052067
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAGEC3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAGEC3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID139081
  • Target name
    MAGEC3
  • Target description
    MAGE family member C3
  • Target synonyms
    CT7.2, HCA2, MAGE-C3, MAGEC4, melanoma-associated antigen C3, MAGE family testis and tumor-specific protein, MAGE-C3 antigen, cancer/testis antigen 7.2, cancer/testis antigen family 7, member 2, hepatocellular carcinoma-associated antigen 2, hepatocellular carcinoma-associated protein HCA2, melanoma antigen family C, 3, melanoma antigen family C3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TD91
  • Protein Name
    Melanoma-associated antigen C3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MAGEC3 using anti-MAGEC3 antibody (A16360-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAGEC3 antigen affinity purified polyclonal antibody (Catalog # A16360-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAGEC3 at approximately 72 kDa. The expected band size for MAGEC3 is at 72 kDa.
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Western blot All lanes: MAGEC3 antibody at 2microg/ml Lane 1: A375 whole cell lysate Lane 2: Rat thymus tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72, 39 kDa Observed band size: 72 kDa
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