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MAGEC3 Antibody

LS-C378187
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMAGEC3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAGEC3 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID139081
  • Target name
    MAGEC3
  • Target description
    MAGE family member C3
  • Target synonyms
    CT7.2, HCA2, MAGE-C3, MAGEC4, melanoma-associated antigen C3, MAGE family testis and tumor-specific protein, MAGE-C3 antigen, cancer/testis antigen 7.2, cancer/testis antigen family 7, member 2, hepatocellular carcinoma-associated antigen 2, hepatocellular carcinoma-associated protein HCA2, melanoma antigen family C, 3, melanoma antigen family C3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAGEC3 using anti-MAGEC3 antibody (A16360-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAGEC3 antigen affinity purified polyclonal antibody (Catalog # A16360-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAGEC3 at approximately 72 kDa. The expected band size for MAGEC3 is at 72 kDa.
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