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Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.

Anti-MAGI2 Antibody

HPA013650
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAGI2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAGI2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9863
  • Target name
    MAGI2
  • Target description
    membrane associated guanylate kinase, WW and PDZ domain containing 2
  • Target synonyms
    ACVRIP1, AIP-1, AIP1, ARIP1, MAGI-2, NPHS15, SSCAM, membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2, activin receptor interacting protein 1, atrophin-1-interacting protein 1, atrophin-1-interacting protein A, membrane-associated guanylate kinase inverted 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UL8
  • Protein Name
    Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MAGI2 using anti-MAGI2 antibody (A04546-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAGI2 antigen affinity purified polyclonal antibody (Catalog # A04546-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAGI2 at approximately 140 kDa. The expected band size for MAGI2 is at 159 kDa.
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