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MAGI2 / AIP-1 Antibody

LS-C671673
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAGI2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAGI2 / AIP-1 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IF, IHC, IHC-P
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9863
  • Target name
    MAGI2
  • Target description
    membrane associated guanylate kinase, WW and PDZ domain containing 2
  • Target synonyms
    ACVRIP1, AIP-1, AIP1, ARIP1, MAGI-2, NPHS15, SSCAM, membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2, activin receptor interacting protein 1, atrophin-1-interacting protein 1, atrophin-1-interacting protein A, membrane-associated guanylate kinase inverted 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAGI2 using anti-MAGI2 antibody (A04546-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAGI2 antigen affinity purified polyclonal antibody (Catalog # A04546-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAGI2 at approximately 140 kDa. The expected band size for MAGI2 is at 159 kDa.
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