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Anti-Major vault protein [1032], Mouse IgG1, kappa

AB04245-1.1
Absolute Antibody
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMVP
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Major vault protein [1032], Mouse IgG1, kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The original version of this antibody was used for an immunohistochemistry experiment on human FFPE tissue blocks (Ramberg et al., 2021; PMID:33609362), on MCF-7 breast cancer cells (Abbondanza et al., 1998; PMID:9628887). Further this antibody was used for immunohistochemistry on cervix carcinoma tissues (Lloret et al., 2009; PMID:19251084), on human lung tissue (Schroeijers et al., 2001; PMID:11668191). Finally, a western blot on human liver tumour and nontumor tissue lysates was performed using this antibody (Megger et al., 2013; PMID:23462207).
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1032
  • Gene ID9961
  • Target name
    MVP
  • Target description
    major vault protein
  • Target synonyms
    LRP, VAULT1, major vault protein, lung resistance-related protein, testicular secretory protein Li 30
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ14764
  • Protein Name
    Major vault protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MVP using anti-MVP antibody (A00642-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVP antigen affinity purified polyclonal antibody (Catalog # A00642-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MVP at approximately 99KD. The expected band size for MVP is at 99KD.
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