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Major Vault Protein (MVP) Antibody

ABX109322
Abbexa
ApplicationsWestern Blot, ELISA
Product group Antibodies
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Overview

  • Supplier
    Abbexa
  • Product Name
    Major Vault Protein (MVP) Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MVP using anti-MVP antibody (A00642-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVP antigen affinity purified polyclonal antibody (Catalog # A00642-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MVP at approximately 99KD. The expected band size for MVP is at 99KD.
Product group Antibodies
Boster Bio
Anti-MVP Antibody Picoband(r)A00642-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetMVP
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Product group Antibodies
Biosynth
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RayBiotech
Anti-MVP Antibody144-01980
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMVP
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Product group Antibodies
Absolute Antibody
Anti-Major vault protein [1032], Mouse IgG1, kappaAB04245-1.1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMVP
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Anti-MVP Antibody
Product group Antibodies
Antibodies.com
Anti-MVP AntibodyA30540
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
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Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (LRP/MVP) polyclonal Antibody, Unconjugated (bs-0661R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Product group Antibodies
References
Bioss
LRP/MVP Polyclonal AntibodyBS-0661R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Zebra Fish
TargetMVP
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Product group Antibodies
Biomatik
MVP Polyclonal AntibodyCAC13838
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetMVP
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Western blot All lanes: MVP antibody at 2microg/ml + Rat lung tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 100 kDa Observed band size: 110 kDa
Product group Antibodies
Cusabio
MVP AntibodyCSB-PA015248LA01HU
ApplicationsWestern Blot, ELISA
ReactivityHuman, Rat
TargetMVP
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Product group Antibodies
LifeSpan BioSciences
MVP / VAULT1 AntibodyLS-C400857
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ReactivityHuman, Mouse, Rat
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Wild-type (WT) and MVP/LRP knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MVP/LRP antibody [C2C3], C-term (GTX100536) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GeneTex
MVP/LRP antibody [C2C3], C-termGTX100536
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetMVP
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