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Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.

Anti-MALSU1 Antibody

HPA020487
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMALSU1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MALSU1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID115416
  • Target name
    MALSU1
  • Target description
    mitochondrial assembly of ribosomal large subunit 1
  • Target synonyms
    C7orf30, mtRsfA, mitochondrial assembly of ribosomal large subunit protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96EH3
  • Protein Name
    Mitochondrial assembly of ribosomal large subunit protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of C7orf30/MALSU1 using anti-C7orf30/MALSU1 antibody (A12786). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C7orf30/MALSU1 antigen affinity purified polyclonal antibody (Catalog # A12786) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C7orf30/MALSU1 at approximately 24 kDa. The expected band size for C7orf30/MALSU1 is at 26 kDa.
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