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Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in glandular cells.

Anti-MAN2C1 Antibody

HPA040627
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAN2C1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAN2C1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4123
  • Target name
    MAN2C1
  • Target description
    mannosidase alpha class 2C member 1
  • Target synonyms
    CDDG2, MAN6A8, MANA, MANA1, alpha-mannosidase 2C1, alpha mannosidase 6A8B, alpha-D-mannoside mannohydrolase, cytosolic alpha-mannosidase, mannosidase, alpha 6A8, mannosidase, alpha A, cytoplasmic, testicular tissue protein Li 115
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NTJ4
  • Protein Name
    Alpha-mannosidase 2C1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in glandular cells.
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Immunohistochemistry analysis of human appendix tissue using CSB-PA878885LA01HU at dilution of 1:100
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Figure 1. Western blot analysis of MAN2C1 using anti-MAN2C1 antibody (A10839). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAN2C1 antigen affinity purified polyclonal antibody (Catalog # A10839) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAN2C1 at approximately 100 kDa. The expected band size for MAN2C1 is at 116 kDa.
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