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MAN2C1 Antibody (HRP)

LS-C501882
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetMAN2C1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAN2C1 Antibody (HRP)
  • Delivery Days Customer
    23
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID4123
  • Target name
    MAN2C1
  • Target description
    mannosidase alpha class 2C member 1
  • Target synonyms
    CDDG2, MAN6A8, MANA, MANA1, alpha-mannosidase 2C1, alpha mannosidase 6A8B, alpha-D-mannoside mannohydrolase, cytosolic alpha-mannosidase, mannosidase, alpha 6A8, mannosidase, alpha A, cytoplasmic, testicular tissue protein Li 115
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAN2C1 using anti-MAN2C1 antibody (A10839). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAN2C1 antigen affinity purified polyclonal antibody (Catalog # A10839) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAN2C1 at approximately 100 kDa. The expected band size for MAN2C1 is at 116 kDa.
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