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Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Anti-MAP2K7 Antibody

HPA001633
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAP2K7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAP2K7
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5609
  • Target name
    MAP2K7
  • Target description
    mitogen-activated protein kinase kinase 7
  • Target synonyms
    JNKK2, MAPKK7, MEK, MEK 7, MKK7, PRKMK7, SAPKK-4, SAPKK4, dual specificity mitogen-activated protein kinase kinase 7, JNK-activating kinase 2, MAP kinase kinase 7, MAPK/ERK kinase 7, SAPK kinase 4, c-Jun N-terminal kinase kinase 2, stress-activated protein kinase kinase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14733
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody (PB9764). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat testis tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK7/MAP2K7 antigen affinity purified polyclonal antibody (Catalog # PB9764) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK7/MAP2K7 at approximately 47 kDa. The expected band size for MEK7/MAP2K7 is at 47 kDa.
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