Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MEK7 (Ser271 + Thr275) Polyclonal Antibody, Unconjugated (bs-3277R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
MKK7 (Ser271 + Thr275) Antibody
BS-3277R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetMAP2K7
Overview
- SupplierBioss Antibodies
- Product NameMKK7 (Ser271 + Thr275) Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID5609
- Target nameMAP2K7
- Target descriptionmitogen-activated protein kinase kinase 7
- Target synonymsc-Jun N-terminal kinase kinase 2; dual specificity mitogen-activated protein kinase kinase 7; JNK-activating kinase 2; JNKK2; MAP kinase kinase 7; MAPK/ERK kinase 7; MAPKK7; MEK; MEK 7; MKK7; PRKMK7; SAPK kinase 4; SAPKK4; SAPKK-4; stress-activated protein kinase kinase 4
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
- Storage Instruction-20°C
- UNSPSC12352203