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Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Anti-MAP3K2 Antibody

HPA021540
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAP3K2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAP3K2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10746
  • Target name
    MAP3K2
  • Target description
    mitogen-activated protein kinase kinase kinase 2
  • Target synonyms
    MAP/ERK kinase kinase 2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2; MEKK2; MEKK2B; mitogen-activated protein kinase kinase kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2U5
  • Protein Name
    Mitogen-activated protein kinase kinase kinase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of MEKK2/MAP3K2 using anti-MEKK2/MAP3K2 antibody (A02354-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2/MAP3K2 antigen affinity purified polyclonal antibody (Catalog # A02354-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2/MAP3K2 at approximately 78KD. The expected band size for MEKK2/MAP3K2 is at 78KD.
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