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MEKK2 Antibody (YA3215, Rabbit)

HY-P83470
MedChem Express
TargetMAP3K2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    MEKK2 Antibody (YA3215, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10746
  • Target name
    MAP3K2
  • Target description
    mitogen-activated protein kinase kinase kinase 2
  • Target synonyms
    MAP/ERK kinase kinase 2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2; MEKK2; MEKK2B; mitogen-activated protein kinase kinase kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MEKK2 Antibody (YA3215) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3215), targeting MEKK2, with a predicted molecular weight of 70 kDa (observed band size: 72 kDa). MEKK2 Antibody (YA3215) can be used for WB, ICC/IF, IP experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MEKK2/MAP3K2 using anti-MEKK2/MAP3K2 antibody (A02354-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2/MAP3K2 antigen affinity purified polyclonal antibody (Catalog # A02354-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2/MAP3K2 at approximately 78KD. The expected band size for MEKK2/MAP3K2 is at 78KD.
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