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Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Anti-MAPK12 Antibody

HPA054562
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAPK12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAPK12 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6300
  • Target name
    MAPK12
  • Target description
    mitogen-activated protein kinase 12
  • Target synonyms
    ERK-6, ERK3, ERK6, MAPK 12, P38GAMMA, PRKM12, SAPK-3, SAPK3, mitogen-activated protein kinase 12, MAP kinase 12, MAP kinase p38 gamma, extracellular signal-regulated kinase 6, mitogen-activated protein kinase 3, mitogen-activated protein kinase p38 gamma, stress-activated protein kinase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53778
  • Protein Name
    Mitogen-activated protein kinase 12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Mouse skeletal muscle tissue All lanes: MAPK12 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 41 kDa Observed band size: 42 kDa
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Figure 1. Western blot analysis of P38 Gamma/MAPK12 using anti-P38 Gamma/MAPK12 antibody (A03942-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P38 Gamma/MAPK12 antigen affinity purified polyclonal antibody (Catalog # A03942-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P38 Gamma/MAPK12 at approximately 42 kDa. The expected band size for P38 Gamma/MAPK12 is at 42 kDa.
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