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ProductsBack/MAPK12 / ERK6 / SAPK3 Antibody

MAPK12 / ERK6 / SAPK3 Antibody

LS-C748111
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetMAPK12
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAPK12 / ERK6 / SAPK3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 40kDa/41kDa, while the observed MW by Western blot was 42kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:2000 - 1:5000) The predicted MW is 40kDa/41kDa, while the observed MW by Western blot was 42kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6300
  • Target name
    MAPK12
  • Target description
    mitogen-activated protein kinase 12
  • Target synonyms
    ERK-6, ERK3, ERK6, MAPK 12, P38GAMMA, PRKM12, SAPK-3, SAPK3, mitogen-activated protein kinase 12, MAP kinase 12, MAP kinase p38 gamma, extracellular signal-regulated kinase 6, mitogen-activated protein kinase 3, mitogen-activated protein kinase p38 gamma, stress-activated protein kinase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P38 Gamma/MAPK12 using anti-P38 Gamma/MAPK12 antibody (A03942-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P38 Gamma/MAPK12 antigen affinity purified polyclonal antibody (Catalog # A03942-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P38 Gamma/MAPK12 at approximately 42 kDa. The expected band size for P38 Gamma/MAPK12 is at 42 kDa.
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