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Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in subset of glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in subset of glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in subset of glandular cells.

Anti-MARS2 Antibody

HPA035589
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMARS2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MARS2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92935
  • Target name
    MARS2
  • Target description
    methionyl-tRNA synthetase 2, mitochondrial
  • Target synonyms
    COXPD25, MetRS, mtMetRS, methionine--tRNA ligase, mitochondrial, methionine tRNA ligase 2, mitochondrial, methionine--tRNA ligase 2, methionine-tRNA synthetase 2, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96GW9
  • Protein Name
    Methionine--tRNA ligase, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of MARS2 using anti-MARS2 antibody (A10675). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARS2 antigen affinity purified polyclonal antibody (Catalog # A10675) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARS2 at approximately 70 kDa. The expected band size for MARS2 is at 70 kDa.
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