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Western Blot Positive WB detected in: K562 whole cell lysate All lanes: MARS2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western Blot Positive WB detected in: K562 whole cell lysate All lanes: MARS2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western Blot Positive WB detected in: K562 whole cell lysate All lanes: MARS2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa

MARS2 Antibody

CSB-PA836221LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMARS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    MARS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92935
  • Target name
    MARS2
  • Target description
    methionyl-tRNA synthetase 2, mitochondrial
  • Target synonyms
    COXPD25, MetRS, mtMetRS, methionine--tRNA ligase, mitochondrial, methionine tRNA ligase 2, mitochondrial, methionine--tRNA ligase 2, methionine-tRNA synthetase 2, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96GW9
  • Protein Name
    Methionine--tRNA ligase, mitochondrial
  • Scientific Description
    ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MARS2 using anti-MARS2 antibody (A10675). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARS2 antigen affinity purified polyclonal antibody (Catalog # A10675) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARS2 at approximately 70 kDa. The expected band size for MARS2 is at 70 kDa.
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