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Anti-MASP2 Antibody

102-26198
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetMASP2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MASP2 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID10747
  • Target name
    MASP2
  • Target description
    MBL associated serine protease 2
  • Target synonyms
    mannan binding lectin serine peptidase 2; mannan-binding lectin serine peptidase 1 pseudogene 1; mannan-binding lectin serine protease 1 pseudogene 1; mannan-binding lectin serine protease 2; mannose-binding lectin associated protein 19; mannose-binding lectin-associated serine protease 2; mannose-binding protein-associated serine protease 2; MAP19; MAP-2; MASP1P1; MASP-2; MBL-associated plasma protein of 19 kD; small MBL-associated protein; sMAP
  • Host
    Rabbit
  • Protein IDO00187
  • Protein Name
    Mannan-binding lectin serine protease 2
  • Scientific Description
    Rabbit Anti-MASP2 Antibody, 100 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MASP2 using anti-MASP2 antibody (A02323-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASP2 antigen affinity purified polyclonal antibody (Catalog # A02323-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MASP2 at approximately 76KD. The expected band size for MASP2 is at 76KD.
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