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Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.

Anti-MASP2 Antibody

HPA029313
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetMASP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MASP2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10747
  • Target name
    MASP2
  • Target description
    MBL associated serine protease 2
  • Target synonyms
    mannan binding lectin serine peptidase 2; mannan-binding lectin serine peptidase 1 pseudogene 1; mannan-binding lectin serine protease 1 pseudogene 1; mannan-binding lectin serine protease 2; mannose-binding lectin associated protein 19; mannose-binding lectin-associated serine protease 2; mannose-binding protein-associated serine protease 2; MAP19; MAP-2; MASP1P1; MASP-2; MBL-associated plasma protein of 19 kD; small MBL-associated protein; sMAP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00187
  • Protein Name
    Mannan-binding lectin serine protease 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MASP2 using anti-MASP2 antibody (A02323-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASP2 antigen affinity purified polyclonal antibody (Catalog # A02323-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MASP2 at approximately 76KD. The expected band size for MASP2 is at 76KD.
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Paraformaldehyde-fixed, paraffin embedded; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with MASP2 Polyclonal Antibody, Unconjugated (bs-1980R)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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