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Immunohistochemical staining of human Prostate shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human Prostate shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human Prostate shows strong nuclear positivity in glandular cells.

Anti-MAT2A-25ul

HPA043028
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAT2A-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC, ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31153
  • Protein Name
    S-adenosylmethionine synthase isoform type-2
  • Scientific Description
    Rabbit Polyclonal Anti-MAT2A Antibody against Human methionine adenosyltransferase II, alpha. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAT2A using anti-MAT2A antibody (A04557-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT2A antigen affinity purified polyclonal antibody (Catalog # A04557-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT2A at approximately 50 kDa. The expected band size for MAT2A is at 44 kDa.
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