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Immunohistochemical staining of human spleen shows strong positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong positivity in cells in red pulp.

Anti-MATK Antibody

HPA004847
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMATK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MATK
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4145
  • Target name
    MATK
  • Target description
    megakaryocyte-associated tyrosine kinase
  • Target synonyms
    CHK; CSK homologous kinase; Csk-homologous kinase; Csk-type protein tyrosine kinase; CTK; hematopoietic consensus tyrosine-lacking kinase; HHYLTK; hydroxyaryl-protein kinase; HYL; HYL tyrosine kinase; HYLTK; leukocyte carboxyl-terminal src kinase related; Lsk; megakaryocyte-associated tyrosine-protein kinase; protein kinase HYL; tyrosine kinase MATK; tyrosine-protein kinase CTK; tyrosylprotein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42679
  • Protein Name
    Megakaryocyte-associated tyrosine-protein kinase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MATK using anti-MATK antibody (A02050-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATK antigen affinity purified polyclonal antibody (Catalog # A02050-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATK at approximately 52 kDa. The expected band size for MATK is at 56 kDa.
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