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Immunofluorescent staining of human cell line K-562 shows localization to cytosol, centriolar satellites & mitotic spindle.
Immunofluorescent staining of human cell line K-562 shows localization to cytosol, centriolar satellites & mitotic spindle.
Immunofluorescent staining of human cell line K-562 shows localization to cytosol, centriolar satellites & mitotic spindle.

Anti-MATK Antibody

HPA066547
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMATK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MATK Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4145
  • Target name
    MATK
  • Target description
    megakaryocyte-associated tyrosine kinase
  • Target synonyms
    CHK, CTK, HHYLTK, HYL, HYLTK, Lsk, megakaryocyte-associated tyrosine-protein kinase, CSK homologous kinase, Csk-homologous kinase, Csk-type protein tyrosine kinase, HYL tyrosine kinase, hematopoietic consensus tyrosine-lacking kinase, hydroxyaryl-protein kinase, leukocyte carboxyl-terminal src kinase related, protein kinase HYL, tyrosine kinase MATK, tyrosine-protein kinase CTK, tyrosylprotein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42679
  • Protein Name
    Megakaryocyte-associated tyrosine-protein kinase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MATK using anti-MATK antibody (A02050-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATK antigen affinity purified polyclonal antibody (Catalog # A02050-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATK at approximately 52 kDa. The expected band size for MATK is at 56 kDa.
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