Anti-MCSP [LC007 (M4-3-ML2)]

Absolute Antibody

Anti-MCSP [LC007 (M4-3-ML2)]

9

This antibody recognizes a membrane-proximal epitope on the D3 domain of human MCSP. It also cross reacts with MCSP from cynomolgus monkey.

The binding characterization of the original mouse version of this antibody was done using ELISA. This antibody can also binds to native form of MCSP expressed on Colo38 cells in a flow cytometric analysis. This antibody can also be used for immunofluorescence staining of MCSP in Colo38 cells. The original mouse version can also detect MCSP fragments in a western blot (Freytag et al., US9309306). The original mouse version of this antibody LC007 is capable of inducing antibody dependent cellular cytotoxicity. This antibody can bind the native epitope on MCSP+ melanoma cells, but there is no induction of internalization. This antibody can be used for immunohistochemical staining of MCSP+ cells in formalin fixed paraffin embedded tissue. It was reported that glycoengineering of LC007 antibody using GlycoMab technology resulted in increased binding affinity for hFcgRIIIa and enhanced ADCC potency and absolute killing of melanoma cell lines. (Moessner et al, Abstract LB-236- AACR 103rd Annual Meeting 2012, Mar 31-Apr 4, 2012; Chicago, IL). The CD16 158 V variant of CD16-CAR T cells showed increased cytotoxic activity against all the tested cancer cells in the presence of the original mouse version of this antibody (PMID: 30429531).

Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

LC007 (M4-3-ML2)

CSPG4

chondroitin sulfate proteoglycan 4

chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan NG2; CSPG4A; HMW-MAA; MCSP; MCSPG; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; MEL-CSPG; MSK16; NG2

Rabbit

IgG

Chondroitin sulfate proteoglycan 4

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human, Monkey

-20°C,2°C to 8°C

12352203