More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-MDM2 Antibody
Anti-MDM2 Antibody

Anti-MDM2 Antibody

A101381
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-MDM2 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to MDM2.
  • Reactivity
    Human
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
MDM2 AntibodyCAB0345
ReactivityHuman
TargetMDM2
  • SizePrice
Western Blot analysis of Hela cells using MDM2 Polyclonal Antibody
Product group Antibodies
Cusabio
MDM2 AntibodyCSB-PA003212
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse
TargetMDM2
  • SizePrice
Product group Antibodies
Biomatik
MDM2 Polyclonal AntibodyCAC15788
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetMDM2
  • SizePrice
Figure 1. Western blot analysis of MDM2 using anti-MDM2 antibody (A00054-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat RH-35 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: mouse NTH/3T3 whole cell lysates, Lane 6: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (Catalog # A00054-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDM2 at approximately 90KD. The expected band size for MDM2 is at 90KD.
Product group Antibodies
Boster Bio
Anti-MDM2 Antibody Picoband(r)A00054-2-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMDM2
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MDM2 Antibody144-60282
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMDM2
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-MDM2 (Ser166) Antibody (YA1888, Rabbit)HY-P82143
TargetMDM2
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-MDM2 (isoform)EB06990
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Feline, Human, Porcine, Rat
TargetMDM2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MDM2 Antibody (C-Terminus)LS-C368433
ApplicationsWestern Blot
ReactivityBovine, Canine, Human, Mouse, Rat
TargetMDM2
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with phospho-MDM2 (Ser166) Polyclonal Antibody, Unconjugated (bs-3266R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
Bioss
Phospho-MDM2 (Ser166) Polyclonal AntibodyBS-3266R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityEquine, Human, Mouse, Rabbit, Rat
TargetMDM2
  • SizePrice
MDM2 antibody detects MDM2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Scale bar= 10μm.
Product group Antibodies
GeneTex
MDM2 antibodyGTX100531
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetMDM2
  • SizePrice