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MDM2 antibody

70R-36777
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    MDM2 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MDM2 using anti-MDM2 antibody (A00054-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat RH-35 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: mouse NTH/3T3 whole cell lysates, Lane 6: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (Catalog # A00054-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDM2 at approximately 90KD. The expected band size for MDM2 is at 90KD.
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Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with phospho-MDM2 (Ser166) Polyclonal Antibody, Unconjugated (bs-3266R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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