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Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.

Anti-MECOM Antibody

HPA046537
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMECOM
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MECOM
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2122
  • Target name
    MECOM
  • Target description
    MDS1 and EVI1 complex locus
  • Target synonyms
    AML1-EVI-1, EVI1, KMT8E, MDS1, MDS1-EVI1, PRDM3, RUSAT2, histone-lysine N-methyltransferase MECOM, AML1-EVI-1 fusion protein, MDS1 and EVI1 complex locus protein EVI1, MDS1 and EVI1 complex locus protein MDS1, PR domain 3, ecotropic virus integration site 1 protein homolog, myelodysplasia syndrome-associated protein 1, oncogene EVI1, zinc finger protein Evi1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03112
  • Protein Name
    MDS1 and EVI1 complex locus protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of EVI1/MECOM using anti-EVI1/MECOM antibody (A04590-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Skov3 whole cell lysates, Lane 2: human Hel whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EVI1/MECOM antigen affinity purified polyclonal antibody (Catalog # A04590-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EVI1/MECOM at approximately 138 kDa. The expected band size for EVI1/MECOM is at 138 kDa.
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