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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.

Anti-MED13 Antibody

HPA067939
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMED13
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MED13 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9969
  • Target name
    MED13
  • Target description
    mediator complex subunit 13
  • Target synonyms
    ARC250, DRIP250, HSPC221, MRD61, THRAP1, TRAP240, mediator of RNA polymerase II transcription subunit 13, activator-recruited cofactor 250 kDa component, mediator of RNA polymerase II transcription, subunit 13 homolog, thyroid hormone receptor-associated protein 1, thyroid hormone receptor-associated protein complex 240 kDa component, thyroid hormone receptor-associated protein complex component TRAP240, thyroid hormone receptor-associated protein, 240 kDa subunit, vitamin D3 receptor-interacting protein complex component DRIP250
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHV7
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 13
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MED13 using anti-MED13 antibody (A04545-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED13 antigen affinity purified polyclonal antibody (Catalog # A04545-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED13 at approximately 300KD. The expected band size for MED13 is at 239KD.
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