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Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Anti-MED28 Antibody

HPA035901
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetMED28
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MED28
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80306
  • Target name
    MED28
  • Target description
    mediator complex subunit 28
  • Target synonyms
    1500003D12Rik; EG1; endothelial-derived gene 1; endothelial-derived protein 1; magicin; mediator of RNA polymerase II transcription subunit 28; mediator of RNA polymerase II transcription, subunit 28 homolog; merlin and Grb2-interacting cytoskeletal protein; tumor angiogenesis marker EG-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H204
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 28
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MED28 using anti-MED28 antibody (A08611-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED28 antigen affinity purified polyclonal antibody (Catalog # A08611-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED28 at approximately 24 kDa. The expected band size for MED28 is at 20 kDa.
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