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anti-MED8 antibody

ARG55394
Arigo Biolaboratories
ApplicationsWestern Blot
Product group Antibodies
TargetMED8
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-MED8 antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID112950
  • Target name
    MED8
  • Target description
    mediator complex subunit 8
  • Target synonyms
    ARC32, mediator of RNA polymerase II transcription subunit 8, activator-recruited cofactor 32 kDa component, mediator of RNA polymerase II transcription subunit MED8
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MED8 using anti-MED8 antibody (A08094-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED8 antigen affinity purified polyclonal antibody (Catalog # A08094-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED8 at approximately 29 kDa. The expected band size for MED8 is at 29 kDa.
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