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Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.

Anti-MEF2C Antibody

AMAB90727
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMEF2C
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MEF2C Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL0368
  • Conjugate
    Unconjugated
  • Gene ID4208
  • Target name
    MEF2C
  • Target description
    myocyte enhancer factor 2C
  • Target synonyms
    C5DELq14.3, DEL5q14.3, NEDHSIL, myocyte-specific enhancer factor 2C, MADS box transcription enhancer factor 2, polypeptide C
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ06413
  • Protein Name
    Myocyte-specific enhancer factor 2C
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MEF2C using anti-MEF2C antibody (A01131-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human COLO320 whole cell lysates, Lane 3: human DAUDI whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEF2C antigen affinity purified polyclonal antibody (Catalog # A01131-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEF2C at approximately 50-65KD. The expected band size for MEF2C is at 50-65KD.
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Immunohistochemical staining of human cerebral cortex shows nuclear immunoreactivity in a subset of neurons.
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