Bio-Connect
Figure 1. Western blot analysis of MEIOB using anti-MEIOB antibody (A13211-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEIOB antigen affinity purified polyclonal antibody (Catalog # A13211-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEIOB at approximately 49 kDa. The expected band size for MEIOB is at 49 kDa.
Figure 1. Western blot analysis of MEIOB using anti-MEIOB antibody (A13211-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEIOB antigen affinity purified polyclonal antibody (Catalog # A13211-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEIOB at approximately 49 kDa. The expected band size for MEIOB is at 49 kDa.
Figure 1. Western blot analysis of MEIOB using anti-MEIOB antibody (A13211-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEIOB antigen affinity purified polyclonal antibody (Catalog # A13211-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEIOB at approximately 49 kDa. The expected band size for MEIOB is at 49 kDa.

Anti-MEIOB Antibody Picoband(r)

A13211-2-CARRIER-FREE
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetMEIOB
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-MEIOB Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID254528
  • Target name
    MEIOB
  • Target description
    meiosis specific with OB-fold
  • Target synonyms
    C16orf73; gs129; meiosis specific with OB domains; meiosis-specific with OB domain-containing protein; SPGF22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N635
  • Protein Name
    Meiosis-specific with OB domain-containing protein
  • Scientific Description
    Boster Bio Anti-MEIOB Antibody Picoband® catalog # A13211-2. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

WB analysis of human lung tissue lysate using GTX45259 C16orf73 antibody, Internal. Dilution : 1microg/ml
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetMEIOB
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
TargetMEIOB
  • SizePrice
Immunofluorescent staining of human cell line SiHa shows localization to nucleus & nucleoli.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetMEIOB
  • SizePrice