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ProductsBack/Anti-MEK3/MAP2K3 Antibody Picoband(r)
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.

Anti-MEK3/MAP2K3 Antibody Picoband(r)

Research Use Only
PA1377
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-MEK3 Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    IHP, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Formulation
    Lyophilized
  • Gene ID5606
  • Target name
    MAP2K3
  • Target description
    mitogen-activated protein kinase kinase 3
  • Target synonyms
    dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; MKK3; PRKMK3; SAPK kinase 2; SAPKK2; SAPKK-2; stress-activated protein kinase kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46734
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 3
  • Scientific Description
    Boster Bio Anti-MEK3/MAP2K3 Antibody catalog # PA1377. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Hamster, Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Hamster
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203