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ProductsBack/Anti-MerTK [Ab2000]

Anti-MerTK [Ab2000]

AB03811-23.0
Absolute Antibody
ApplicationsELISA, Neutralisation/Blocking
Product group Antibodies
TargetMERTK
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-MerTK [Ab2000]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of the IgG1 format of the antibody was confirmed by ELISA analysis. The antibody bound to cellularly expressed full length human MerTK in a dose responsive manner, with EC50 values in the picomolar to nanomolar range, as determined by FACS. The antibody inhibited Gas6 binding to hMerTK in a dose responsive manner, with an IC50 of 1 nM (WO2019084307A1).
  • Applications
    ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Ab2000
  • Gene ID10461
  • Target name
    MERTK
  • Target description
    MER proto-oncogene, tyrosine kinase
  • Target synonyms
    MER, RP38, Tyro12, c-Eyk, c-mer, tyrosine-protein kinase Mer, MER receptor tyrosine kinase, STK kinase, c-mer proto-oncogene tyrosine kinase, proto-oncogene c-Mer, receptor tyrosine kinase MerTK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12866
  • Protein Name
    Tyrosine-protein kinase Mer
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MERTK using anti-MERTK antibody (A00489-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat lung tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MERTK antigen affinity purified polyclonal antibody (Catalog # A00489-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MERTK at approximately 120 kDa. The expected band size for MERTK is at 120 kDa.
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