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Western Blot analysis of HEPG2 cells using MerTK Polyclonal Antibody
Western Blot analysis of HEPG2 cells using MerTK Polyclonal Antibody
Western Blot analysis of HEPG2 cells using MerTK Polyclonal Antibody

MERTK Antibody

CSB-PA003228
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMERTK
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Overview

  • Supplier
    Cusabio
  • Product Name
    MERTK Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10461
  • Target name
    MERTK
  • Target description
    MER proto-oncogene, tyrosine kinase
  • Target synonyms
    c-Eyk; c-mer; c-mer proto-oncogene tyrosine kinase; MER; MER receptor tyrosine kinase; proto-oncogene c-Mer; receptor tyrosine kinase MerTK; RP38; STK kinase; Tyro12; tyrosine-protein kinase Mer
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12866
  • Protein Name
    Tyrosine-protein kinase Mer
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MERTK using anti-MERTK antibody (A00489-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat lung tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MERTK antigen affinity purified polyclonal antibody (Catalog # A00489-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MERTK at approximately 120 kDa. The expected band size for MERTK is at 120 kDa.
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