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Immunohistochemical staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.

Anti-MESDC2 Antibody

HPA039414
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMESD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MESDC2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23184
  • Target name
    MESD
  • Target description
    mesoderm development LRP chaperone
  • Target synonyms
    BOCA, MESDC2, OI20, LRP chaperone MESD, LDLR chaperone MESD, mesoderm development LRP chaperone MESD, mesoderm development candidate 2, mesoderm development protein, renal carcinoma antigen NY-REN-61
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14696
  • Protein Name
    LRP chaperone MESD
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MESDC2/MESD using anti-MESDC2/MESD antibody (A32230-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MESDC2/MESD antigen affinity purified polyclonal antibody (Catalog # A32230-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MESDC2/MESD at approximately 24 kDa. The expected band size for MESDC2/MESD is at 26 kDa.
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Western blot analysis using Anti-MESDC2 antibody HPA041721 (A) shows similar pattern to independent antibody HPA039414 (B).
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