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WB analysis of various sample lysates using GTX66225 MESDC2 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66225 MESDC2 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66225 MESDC2 antibody. Dilution : 1:3000 Loading : 25microg per lane

MESDC2 antibody

GTX66225
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetMESD
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Overview

  • Supplier
    GeneTex
  • Product Name
    MESDC2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23184
  • Target name
    MESD
  • Target description
    mesoderm development LRP chaperone
  • Target synonyms
    BOCA, MESDC2, OI20, LRP chaperone MESD, LDLR chaperone MESD, mesoderm development LRP chaperone MESD, mesoderm development candidate 2, mesoderm development protein, renal carcinoma antigen NY-REN-61
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14696
  • Protein Name
    LRP chaperone MESD
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MESDC2/MESD using anti-MESDC2/MESD antibody (A32230-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MESDC2/MESD antigen affinity purified polyclonal antibody (Catalog # A32230-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MESDC2/MESD at approximately 24 kDa. The expected band size for MESDC2/MESD is at 26 kDa.
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