Anti-Mesothelin [MB]

Absolute Antibody

Anti-Mesothelin [MB]

9

This antibody can recognize and bind human mesothelin. This antibody binds a different antibody on the human mesothelin than another antibody called MN-1. Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein highly expressed

The binding characterization of this antibody was done using ELISA and SPR. The binding affinity of the original IgG2a antibody for mesothelin-Fc protein was determined by BIACore and reported to be Kd = 0.6 nM. This antibody can recognize membrane bound mesothelin on the surface of mesothelin positive H226, A1847, Hela and Panc3.014 cells (mesothelin-positive) in a flow cytometry. This antibody can also bind denatured human mesothelin in a western blot assay. This antibody was also used in the immunohistochemical staining of cancerous tissue like ovarian adenocarcinoma and normal tissues like peritoneal mesothelium on uterus, pleural mesothelium on lung and fallopian tube luminal fluid (PMID: 16115924). This antibody was used in the development of an ELISA for detection of serum mesothelin levels in healthy volunteers and random hospitalized patients with mesothelioma and ovarian cancer (PMID: 16428485).

Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

MB

MSLN

mesothelin

CAK1 antigen; megakaryocyte potentiating factor; mesothelin; MPF; pre-pro-megakaryocyte-potentiating factor; SMRP; soluble MPF mesothelin related protein

Mouse

IgG2a

Mesothelin

Human

-20°C,2°C to 8°C

12352203