
Figure 1. Western blot analysis of GRM5 using anti-GRM5 antibody (A01338-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM5 antigen affinity purified polyclonal antibody (Catalog # A01338-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRM5 at approximately 150KD. The expected band size for GRM5 is at 132KD.
Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband(r)
A01338-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGRM5
Overview
- SupplierBoster Bio
- Product NameAnti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID2915
- Target nameGRM5
- Target descriptionglutamate metabotropic receptor 5
- Target synonymsglutamate receptor, metabotropic 5; GPRC1E; metabotropic glutamate receptor 5; mGlu5; MGLUR5; PPP1R86; protein phosphatase 1, regulatory subunit 86
- HostRabbit
- IsotypeIgG
- Protein IDP41594
- Protein NameMetabotropic glutamate receptor 5
- Scientific DescriptionBoster Bio Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody catalog # A01338-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203