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mGluR5 antibody [HL2543]

GTX638912
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGRM5
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Overview

  • Supplier
    GeneTex
  • Product Name
    mGluR5 antibody [HL2543]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2543
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2915
  • Target name
    GRM5
  • Target description
    glutamate metabotropic receptor 5
  • Target synonyms
    glutamate receptor, metabotropic 5; GPRC1E; metabotropic glutamate receptor 5; mGlu5; MGLUR5; PPP1R86; protein phosphatase 1, regulatory subunit 86
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41594
  • Protein Name
    Metabotropic glutamate receptor 5
  • Scientific Description
    This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRM5 using anti-GRM5 antibody (A01338-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM5 antigen affinity purified polyclonal antibody (Catalog # A01338-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRM5 at approximately 150KD. The expected band size for GRM5 is at 132KD.
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