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Anti-Methylmalonyl Coenzyme A mutase Antibody

Anti-Methylmalonyl Coenzyme A mutase Antibody

A14496
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-Methylmalonyl Coenzyme A mutase Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to Methylmalonyl Coenzyme A mutase.
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: MMUT antibody at 1:1000 Lane 1: Mouse liver tissue Lane 2: Rat kidney tissue Lane 3: Rat heart tissue Secondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 83 KDa Observed band size: 83 KDa Exposure time: 5min
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Figure 1. Western blot analysis of MUT using anti-MUT antibody (A01065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: human K562 whole cell lysates, lane 2: huam CACO-2 whole cell lysates, lane 3: rat liver tissue lysates, lane 4: rat kidney tissue lysates, lane 5: rat heart tissue lysates, lane 6: mouse liver tissue lysates, lane 7: mouse kidney tissue lysates, lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUT antigen affinity purified polyclonal antibody (Catalog # A01065) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUT at approximately 83KD. The expected band size for MUT is at 83KD.
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