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Methylmalonyl Coenzyme A mutase Antibody (YA295, Rabbit)

HY-P80222
MedChem Express
TargetMMUT
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Methylmalonyl Coenzyme A mutase Antibody (YA295, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4594
  • Target name
    MMUT
  • Target description
    methylmalonyl-CoA mutase
  • Target synonyms
    MCM; methylmalonyl Coenzyme A mutase; methylmalonyl-CoA isomerase; methylmalonyl-CoA mutase c.*192delA; methylmalonyl-CoA mutase c.*51C>G; methylmalonyl-CoA mutase variant c.1495G>A; methylmalonyl-CoA mutase variant c.2011A>G; methylmalonyl-CoA mutase variant c.2150G>T; methylmalonyl-CoA mutase variant c.322C>T; methylmalonyl-CoA mutase variant c.613_615delGAA; methylmalonyl-CoA mutase variant c.636G>A; methylmalonyl-CoA mutase variant c.643G>A; methylmalonyl-CoA mutase, mitochondrial; MUT; mutant methylmalonyl CoA mutase; truncated methylmalonyl CoA mutase; truncated methylmalonyl-CoA mutase variant c.1420C>T; truncated methylmalonyl-CoA mutase variant c.2179C>T; truncated methylmalonyl-CoA mutase variant c.91C>T
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Methylmalonyl Coenzyme A mutase Antibody (YA295) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Methylmalonyl Coenzyme A mutase.
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: MMUT antibody at 1:1000 Lane 1: Mouse liver tissue Lane 2: Rat kidney tissue Lane 3: Rat heart tissue Secondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 83 KDa Observed band size: 83 KDa Exposure time: 5min
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Figure 1. Western blot analysis of MUT using anti-MUT antibody (A01065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: human K562 whole cell lysates, lane 2: huam CACO-2 whole cell lysates, lane 3: rat liver tissue lysates, lane 4: rat kidney tissue lysates, lane 5: rat heart tissue lysates, lane 6: mouse liver tissue lysates, lane 7: mouse kidney tissue lysates, lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUT antigen affinity purified polyclonal antibody (Catalog # A01065) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUT at approximately 83KD. The expected band size for MUT is at 83KD.
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