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Immunohistochemical staining of human endometrium shows nuclear immunoreactivity in glandular cells and in stroma.
Immunohistochemical staining of human endometrium shows nuclear immunoreactivity in glandular cells and in stroma.
Immunohistochemical staining of human endometrium shows nuclear immunoreactivity in glandular cells and in stroma.

Anti-METTL14 Antibody

AMAB91276
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMETTL14
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-METTL14 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL4254
  • Conjugate
    Unconjugated
  • Gene ID57721
  • Target name
    METTL14
  • Target description
    methyltransferase 14, N6-adenosine-methyltransferase non-catalytic subunit
  • Target synonyms
    hMETTL14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, N6-adenosine-methyltransferase non-catalytic subunit, N6-adenosine-methyltransferase subunit METTL14, methyltransferase 14, N6-adenosine-methyltransferase subunit, methyltransferase like 14, methyltransferase-like protein 14
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9HCE5
  • Protein Name
    N(6)-adenosine-methyltransferase non-catalytic subunit METTL14
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of METTL14 using anti-METTL14 antibody (A07389-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL14 antigen affinity purified polyclonal antibody (Catalog # A07389-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METTL14 at approximately 65KD. The expected band size for METTL14 is at 65KD.
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