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Western Blot Positive WB detected in: SH-SY5Y whole cell lysate All lanes: METTL14 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate All lanes: METTL14 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate All lanes: METTL14 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa

METTL14 Antibody

CSB-PA884519LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMETTL14
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Overview

  • Supplier
    Cusabio
  • Product Name
    METTL14 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57721
  • Target name
    METTL14
  • Target description
    methyltransferase 14, N6-adenosine-methyltransferase subunit
  • Target synonyms
    hMETTL14; methyltransferase like 14; methyltransferase-like protein 14; N6-adenosine-methyltransferase non-catalytic subunit; N6-adenosine-methyltransferase subunit METTL14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCE5
  • Protein Name
    N6-adenosine-methyltransferase non-catalytic subunit
  • Scientific Description
    N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5-[AG]GAC-3 consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of METTL14 using anti-METTL14 antibody (A07389-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL14 antigen affinity purified polyclonal antibody (Catalog # A07389-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METTL14 at approximately 65KD. The expected band size for METTL14 is at 65KD.
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