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Immunohistochemical staining of human gallbladder shows weak cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows weak cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows weak cytoplasmic positivity in glandular cells.

Anti-MFF Antibody

HPA010968
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetMFF
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MFF
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56947
  • Target name
    MFF
  • Target description
    mitochondrial fission factor
  • Target synonyms
    C2orf33; EMPF2; GL004; mitochondrial fission factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZY8
  • Protein Name
    Mitochondrial fission factor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate All lanes: MFF antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 33 28, 26 kDa Observed band size: 35 kDa
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Figure 1. Western blot analysis of MFF using anti-MFF antibody (A02563-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human MOLT-4 whole clel lysates, Lane 5: human HEL whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFF antigen affinity purified polyclonal antibody (Catalog # A02563-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFF at approximately 26 kDa. The expected band size for MFF is at 26 kDa.
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